Study on the distribution of sub-genotype B on hepatitis B virus in patients with chronic HBV infection from 4 cities of China / 中华流行病学杂志
Chinese Journal of Epidemiology
;
(12): 52-54, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-287820
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the distribution of sub-genotype B on hepatitis B virus (HBV) in patients with HBV chronic infection from 4 cities (Beijing, Shijiazhuang, Wenzhou and Shenzhen) of China.</p><p><b>METHODS</b>A type-specific nested polymerase chain reaction(PCR) with multiplex pairs of primers was used for HBV genotyping,and Ba and Bj sub-genotypes were identified by a PCR-restriction fragment length polymorphism (PCR-RFLP) method. A total of 101 serum samples collected from patients with chronic HBV/B infection were detected. Among them, 18 were collected from Beijing, 22 from Shijiazhuang, 34 from Wenzhou and 27 from Shenzhen. Thirty from the 101 serum samples were randomly selected and analyzed by PCR product sequencing.</p><p><b>RESULTS</b>All of the 101 serum samples were identified as sub-genotype Ba,and none of them was sub-genotype Bj.</p><p><b>CONCLUSION</b>Sub-genotype Ba was a predominant strain of HBV/B in patients with chronic HBV infection from these 4 cities.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Virologia
/
Polimorfismo de Fragmento de Restrição
/
China
/
Vírus da Hepatite B
/
Reação em Cadeia da Polimerase
/
Classificação
/
Genética
/
Genótipo
/
Hepatite B
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Epidemiology
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS