Effect of zedoary oil for cat D and cat K expression in A549 cell line / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To explore the Zedoary oil on A549 cell line of collagen deposition cat D and cat K expression.</p><p><b>METHOD</b>The A549 cell line were treat by Zedoary oil on four different concentrations (0, 40, 80, 120 mg x L(-1)) in different time. Dynamic changes of collagen in A549 cell using Picric-sirius red method. Cat D and Cat K expression of level were detected by using western blot.</p><p><b>RESULT</b>The collagen content showed that Zedoary oil had an inhibitory effect on the deposition of A549 cells. The results of western blot showed that the expression of cat D and cat K were up-regulated significangly in A549 cells of Zedoary oil groups compared with that in controls.</p><p><b>CONCLUSION</b>A549 cell of collagen deposition were reduced by Zedoary oil. The effects may due to the up-regulation of cat D and cat K.</p>