Study on seeds dormancy release and physiological change of Thesium chinense / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the seeds dormancy release and physiological change of Thesium chinense.</p><p><b>METHOD</b>To release dormancy, the seeds of T. chinense were treated with chemical reagent and stratification under 3-5 degrees C.</p><p><b>RESULT</b>When washed with flowing water for 24 h, then soaked in 500 mg x L(-1) GA3 for 24 h, finally, treated with stratification method under 3-5 degrees C for 150 day, the split rate of T. chinense seeds reached 22%, crude fat decreased 50%, total sugar and dissolvability sugar increased 3-4 times, ABA decreased more than 90%, GA3 increased more than 5 times.</p><p><b>CONCLUSION</b>The seeds dormancy of T. chinense can be released with the method of washing with flowing water for 24 h, then soaking in 500 mg x L(-1) GA3 for 24 h, finally, treated with stratification method under 3-5 degrees C for 150-180 day.</p>