Molecular identification of Ca2+ channels in human ejaculated sperm / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To identify the types of voltage-dependent Ca2+ channels (VDCCs) in human ejaculated sperm using molecular technology.</p><p><b>METHODS</b>Based on the WHO criteria, the donated semen was screened by computer-aided sperm analysis (CASA), the sperm optimized by swim-up method, and the VDCCs' alpha1 subunits examined by reverse transcription polymerase chain reaction (RT-PCR).</p><p><b>RESULTS</b>Such mRNA messages as alpha1H, alpha1G, alpha1E, alpha1B and alpha1C were detected, but alpha1A and alpha1D were not.</p><p><b>CONCLUSION</b>Human motile sperms express multiple VDCC RNAs, among which T-type and non-L-type channel messages are likely to be predominantly expressed. Not only T-type but also non-L-type calcium channels may be major gates for the external calcium influx, which helps regulate sperm motility and acrosome reaction.</p>