Characterization of the mRNA profile in ejaculated spermatozoa from healthy fertile men / 中华男科学杂志
National Journal of Andrology
;
(12): 900-903, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-289114
ABSTRACT
<p><b>OBJECTIVE</b>To explore the complexity of mRNA in the ejaculated sperm from healthy fertile men.</p><p><b>METHODS</b>Semen samples were collected from 10 healthy fathers. The swim-up method was adopted to purify the sperm from possible contamination of somatic cells and the spermatozoal total RNA extracted by Trizol was used for SAGE library analysis.</p><p><b>RESULTS</b>A totle of 21 052 SAGE raw tags were sequenced from 877 clones and 2 712 unique tags that occurred at least twice in the library were given further analysis. 19.7% of the unique tags had no match in the existing SAGE map, representing novel genes. Molecular function analysis revealed 67% of unique tags related to protein binding or nucleic acid binding categories, 41% to catalytic activity, 13% to message transducer activity, and 10% to transporter, structural and transcription regulator activity, respectively.</p><p><b>CONCLUSION</b>There exists a complex repertoire of mRNAs in the ejaculated spermatozoa from fertile men.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Espermatozoides
/
RNA Mensageiro
/
Etiquetas de Sequências Expressas
/
Perfilação da Expressão Gênica
/
Ejaculação
/
Genética
Limite:
Adulto
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2006
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS