Effects of burn serum on the viscoelasticity and the structure of rat intestinal epithelial cells / 中华烧伤杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the effects of burn serum on the viscoelasticity and the structure of rat intestinal epithelial cells.</p><p><b>METHODS</b>The rat intestinal epithelial cell strain (IEC-6) was cultured and stimulated by burn serum. The changes of IECs before and after the stimulation were dynamically observed by cytoskeleton immunohistochemistry, ELISA and the measurement of cytomembranous viscoelasticity.</p><p><b>RESULTS</b>During the early stage of burn serum stimulation, the skeleton protein expression in IEC decreased obviously with weakened positive signals of microfilaments and microtubules and with decreased cellular elasticity.</p><p><b>CONCLUSION</b>The cytoskeleton injury could cause the increase of cellular fragility and the decrease of the viscoelasticity, which ultimately lead to the change of cellular biodynamics. These changes might directly participate the development of postburn intestinal epithelial injury.</p>