An experimental study on the role of indirect antigen presentation pathway on the immunogenetic of epidermal cells / 中华烧伤杂志
Chinese Journal of Burns
;
(6): 15-17, 2003.
Artigo
em Chinês
| WPRIM
| ID: wpr-289149
ABSTRACT
<p><b>OBJECTIVE</b>To explore the role of indirect antigen presentation pathway on the immunogenecity of epidermal cells.</p><p><b>METHODS</b>Human epidermal cells (HEC), allogeneic human peripheral blood lymphocytes (PBL) and mononuclear cells (PBM, including monocytes) were isolated and cultured in vitro. HECs were transfected by human-originated CTLA4Ig-adenovirus vector. The CTLA4Ig expression was observed. Allogeneic PBLs or PBMs were added to the transfected and non-transfected HECs with simple cultured PBLs and PBMs as the control. The proliferation of PBL and PBM was determined by (3)H-TdR incooperation.</p><p><b>RESULTS</b>HECs could be successfully transfected by CTLA4Ig-adenovirus vector and expressed corresponding proteins. The non-transfected HECs could stimulate slight proliferation of allogeneic PBLs (P < 0.05) and stimulate remarkable proliferation of PBMs (including monocytes) (P < 0.05). The proliferation reaction of PBLs and PBMs decreased significantly (P < 0.05) after being stimulated by HEC which was modulated by CTLA4Ig genes.</p><p><b>CONCLUSION</b>Indirect antigen presentation pathway might play important roles in the HEC immunogenicity which could be evidently inhibited by CTLA4Ig.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Leucócitos Mononucleares
/
Linfócitos
/
Transfecção
/
Transdução de Sinais
/
Antígenos de Diferenciação
/
Antígenos CD
/
Divisão Celular
/
Células Cultivadas
/
Adenoviridae
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Burns
Ano de publicação:
2003
Tipo de documento:
Artigo
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