Changes of the mRNA expression and the intestinal mucosal cyclooxygenase activity in scalded rats / 中华烧伤杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the changes of the mRNA expression and the intestinal mucosal cyclooxygenase (COXs) activity in scalded rats.</p><p><b>METHODS</b>Wistar rats inflicted with 30% TBSA III degree scalding were employed as the model. The changes of COX-1, COX-2 activities were determined by substrate fluorescence analysis and the mRNA expressions of COX-1 and COX-2 by RT-PCR.</p><p><b>RESULTS</b>The mRNA expressions and the activities of COX-2 in rat intestinal mucosa increased obviously after injury. But those of COX-1 exhibited lower range of change.</p><p><b>CONCLUSION</b>The pathological mechanism of rat intestinal mucosa injury after scalding might be closely related to the COXs participation by different styles between the two enzymes.</p>