Simvastatin induced apoptosis and its effect on apoptosis-related gene expression in rat vascular smooth muscle cell / 浙江大学学报·医学版
Journal of Zhejiang University. Medical sciences
;
(6): 11-14, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-289334
ABSTRACT
OBJECTIVE:
To observe the influence of simvastatin on the apoptosis of vascular smooth muscle cells (VSMC) and its effects on the expression of apoptosis-related genes.METHODS:
The presence of apoptosis was detected by electron microscope and flow cytometry assessment of PI/Annexin V stain; The protein levels of Bax, Bel-2 and activation of caspase-3 were examined using Western blot technique.RESULTS:
After treatment with 30 &mgr;mol/L simvastatin for 24 h, apoptosis were identified with electron microscope in VSMC and flow cytometry showed that rate of apoptosis in simvastatin group (35.5+/-5.8)% was singificantly higher than that in control group (15.1+/-5.0)%. Western blot analyses revealed that the apoptosis process was associated with upregulation of Bax protein and activation of caspase-3, but not with Bel-2 expression.CONCLUSION:
Simvastatin can induce apoptosis in VSMC in associated with induction of bax and activation of caspase-3.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Journal of Zhejiang University. Medical sciences
Ano de publicação:
2002
Tipo de documento:
Artigo
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