Analysis on chloroplast DNA sequences of Polygonum capitatum of different geographical population / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To investigate the relationship between the variation of chloroplast DNA gene sequences and the geographical origins of Polygonum capitatum in order to provide the molecular evidence for its excellent germplasm resources.</p><p><b>METHOD</b>PCR direct sequencing was applied to detect the chloroplast psbA-trnH, trnL-trnF gene sequence of 11 samples collected from 11 populations of P. capitatum.</p><p><b>RESULT</b>The psbA-trnH gene sequence of P. capitatum from different populations was 402 bp in length, there were 6 variable sites. TrnL-F gene sequence was 875 bp, there were 5 variable sites. The clusters diagram by UPGMA method showed that P. capitatum groups in Yunnan and Guizhou existed a considerable variation.</p><p><b>CONCLUSION</b>P. capitaturni which is located in the east of Yunnan and the west of Guizhou is helpful of screening the germplasm resources.</p>