Instantaneous expression aFGF-GFP fusion gene in safflower / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 281-284, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-289384
ABSTRACT
<p><b>OBJECTIVE</b>To study the instantaneous expression aFGF-GFP fusion gene in Carthamus tinctorius.</p><p><b>METHOD</b>Molecular biology methods were applied to construct aFGF and GFP fusion gene vector, it is transformed into C. tinctorius by Agrobacterium tumefaciens, forming the resistant callus, fluorescence microscopy was used for detection.</p><p><b>RESULT</b>aFGF gene and GFP gene were amplified by PCR reaction. It was successfully constructed plant fluorescence expression vector pCAMBIA139035SaFGF-GFP, it was used to transform C. tinctorius, and the acquired resistance calli showed strong green fluorescence under UV light.</p><p><b>CONCLUSION</b>The expression of GFP in resistance C. tinrictorius calli is good, it is indicated that aFGF gene in plant cells has also been expressed.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Proteínas Recombinantes de Fusão
/
Expressão Gênica
/
Clonagem Molecular
/
Carthamus tinctorius
/
Proteínas de Fluorescência Verde
/
Fatores de Crescimento de Fibroblastos
/
Genética
/
Metabolismo
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2011
Tipo de documento:
Artigo
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