Study on fragmentation of vitexin and isorhamnetin-3-O-beta-D-rutinoside using electrospray quadrupole time of flight mass spectrometry / 中国中药杂志
China Journal of Chinese Materia Medica
; (24): 180-184, 2011.
Article
em Zh
| WPRIM
| ID: wpr-289403
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ABSTRACT
<p><b>OBJECTIVE</b>To investigate the fragmentation pathway of vitexin and isorhamnetin-3-O-beta-D-rutinoside with CID-TOF-MS.</p><p><b>METHOD</b>Equipped with an LC-MS was carried out using an ultra-performance liquid chromatography, electrospray ionization quadrupole collision-induced dissociation-TOF-MS.</p><p><b>RESULT</b>ESI-MS spectrum showed [M-H]- base peak of m/z 431. 0958 and m/z 623.1566. The CID-MS of vitexin showed five basic fragment ions, three of which corresponded to the glucosyl ring fracture: m/z 353, 341 and 311; other two were benzyl ion m/z 283, aglycone ion m/z 269. In addition, two low abundance ions, namely, m/z 161 and m/z 117, generated by RDA cracking ions, were also characteristic ions. The CID-MS of isorhamnetin-3-O-beta-D-rutinoside showed six main characteristic fragments ions corresponding to the loss of rhamnosyl m/z 477 and the glycosyl ring fracture: m/z 387, 357 and 311, and aglycone ion m/z 315. In addition, B ring generated m/z 300 and m/z 271 and C ring generated m/z 243 and the RDA cleavage generated m/z 151 and m/z 125.</p><p><b>CONCLUSION</b>Those fragment ions can be used to quickly identify vitexin and isorhamnetin-3-O-beta-D-rutinoside.</p>
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Índice:
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Assunto principal:
Flavonoides
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Medicamentos de Ervas Chinesas
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Estrutura Molecular
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Química
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Espectrometria de Massas por Ionização por Electrospray
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Apigenina
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Dissacarídeos
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Métodos
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2011
Tipo de documento:
Article