Optimization of extraction procedure of tongmai granules by orthogonal design with pharmacodynamic index / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To optimize the extraction technology for anti-myocardial ischemia active component group in tongmai granules.</p><p><b>METHOD</b>The extracting conditions were optimized by using orthogonal experiment and pharmacodynamic index. Employing puerarin, ferulic acid, tanshinone IIA, and salvianolic acid B as indexes, the effects of ethanol concentration, solvent volume, extraction times and extraction time on the extracting process were investigated.</p><p><b>RESULT</b>The optimum extraction process was adding 8 times amount of 70% alcohol, refluxing and extracting for 3 times, 1.5 h each time.</p><p><b>CONCLUSION</b>This extraction process shows good stability and is available for extracting anti-myocardial ischemia component group in tongmnai granules.</p>