Determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extractive of Wendan formula by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To develop a RP-HPLC method for simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extraction of Wendan formula.</p><p><b>METHOD</b>DIKMA Diamonsil(2)-C18 column (4.6 mm x 250 mm, 5 microm) was used at 25 degrees C with the mobile phase of acetonitrile-0.1% phosphatic acid in a gradient manner. The flow rate was set at 1.0 mL min(-1). The detection wavelength was 237, 283 nm.</p><p><b>RESULT</b>The linear responses ranged from 0.0199-0.1191 microg for liquiritin (r = 0.9997, n = 6), 0.1800-1.0800 microg for naringin (r = 0.9997, n = 5), 0.1455-0.8730 microg for hesperidin (r = 0.9998, n = 6), 0.0393-0.2355 microg for monoammonium glycyrrhizinate (r = 0.9997, n = 6), respectively. The average recoveries were 97.7% with RSD 1.5% for liquiritin, 97.7% with RSD 2.0% for naringin, 97.1% with RSD 2.0% for hesperidin and 98.5% with RSD 1.9% for glycyrrhizic acid, respectively.</p><p><b>CONCLUSION</b>The method is quick, simple and repeatable for simultaneous determination of liquiritin, naringin, hesperidin and glycyrrhizic acid in extraction of Wendan formula.</p>