Forward genetic screening for zebrafish mutants defective in myelopoiesis / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1230-1233, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-289954
ABSTRACT
<p><b>OBJECTIVE</b>To identify zebrafish mutants with myelopoiesis defects by ENU mutagenesis and large-scale forward genetic screening.</p><p><b>METHODS</b>Male zebrafish were mutagenized with N-ethyl N-nitrosourea to induce mutations in the spermatogonial cells to generate the founders, which were outcrossed with AB to raise F1 fish. The F1 fish from different founders were mated to generate the F2 families. The F3 embryos from F2 sibling crosses were screened by Sudan black B staining and neutral red staining.</p><p><b>RESULTS</b>A total of 350 F2 families from F1 sibling crosses were screened, and 1424 F2 crosses were analyzed. Six mutations were identified resulting in abnormal Sudan black B staining and neutral red staining, indicating the involvement of neutrophil deficiency or macrophage abnormalities.</p><p><b>CONCLUSION</b>It is simple and cheap to induce and screen myelopoiesis deficiency in zebrafish by ENU chemical mutagenesis and Sudan black B staining and neutral red staining. These mutants shed light on the identification of the genes important to myelopoiesis in zebrafish.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fisiologia
/
Peixe-Zebra
/
Testes Genéticos
/
Mutagênese
/
Regulação da Expressão Gênica no Desenvolvimento
/
Células Progenitoras Mieloides
/
Mielopoese
/
Genética
/
Mutação
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
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