Hath1 gene transfer inhibits the proliferation of colonic cancer cells in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 1005-1008, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-289980
ABSTRACT
<p><b>OBJECTIVE</b>To study the effect of Hath1 gene transfer on the proliferation of colonic cancer cells in vitro.</p><p><b>METHODS</b>The recombinant plasmid pcDNA3.1(+)-Hath1 was transfected into HT29 colonic cancer cells, and 3 positive cell clones were randomly selected to test the levels of Hath1 mRNA, Muc2 mRNA, Hath1, Muc2, cyclin D1 and p27 by quantitative real-time RT-PCR and Western blotting. The proliferation of the transfected HT29 cells was observed by means of colony formation assay and xenograft growth in nude mice.</p><p><b>RESULTS</b>Hath1 significantly down-regulated of cyclin D1 and up-regulate of p27 expressions and inhibited the proliferation of HT29 cells.</p><p><b>CONCLUSION</b>Hath1 gene may be an anti-oncogene in colon carcinogenesis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
RNA Mensageiro
/
Neoplasias do Colo
/
Técnicas de Transferência de Genes
/
Antígeno Nuclear de Célula em Proliferação
/
Ciclina D1
/
Linhagem Celular Tumoral
/
Proliferação de Células
/
Fatores de Transcrição Hélice-Alça-Hélice Básicos
/
Mucina-2
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS