Dimethylsulfoxide-induced expression of thymic stromal lymphopoietin in human bronchial epithelial cells / 南方医科大学学报
Journal of Southern Medical University
;
(12): 963-965, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-290019
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of dimethylsulfoxide on the expression of thymic stromal lymphopoietin (TSLP) in human bronchial epithelial cell (HBE).</p><p><b>METHODS</b>16HBE cells were incubated in the presence of dimethylsulfoxide at different concentrations, and the cell proliferation changes were observed. The expressions of TSLP mRNA and protein in the cells were detected by real-time quantitative PCR and ELISA, respectively.</p><p><b>RESULTS</b>Dimethylsulfoxide induced significantly increased TSLP mRNA expression in HBE cells (P<0.01) in a concentration-dependent manner. The level of TSLP protein in the supernatant was also increased after dimethylsulfoxide treatment, but high concentration of dimethylsulfoxide resulted in e inhibited cell proliferation.</p><p><b>CONCLUSION</b>Dimethylsulfoxide may affect the immunomodulatory function of HBE cells.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Brônquios
/
RNA Mensageiro
/
Células Cultivadas
/
Dimetil Sulfóxido
/
Citocinas
/
Biologia Celular
/
Proliferação de Células
/
Células Epiteliais
/
Genética
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2010
Tipo de documento:
Artigo
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