Investigation on vibrio cholera carried in aquatic products of littoral areas, Zhejiang Province / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 336-338, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-290263
ABSTRACT
<p><b>OBJECTIVE</b>To examine vibrio cholera (V.C) in aquatic products of littoral area, Zhejiang Province and to provide scientific evidence for administration of aquatic products and cholera epidemic control.</p><p><b>METHODS</b>All 990 samples of aquatic products collected from local markets, eateries and aquafarms in three chosen areas. Samples were proliferated in alkaline liquid medium, and purified in NO 4 medium, the isolations were identified biochemically, and phenotype of strains were defined by phagocyte and coagulation with V.C. diagnostic serum. Three virulence genes (ctx, ace, zct) of the isolated strains were detected by polymerase chain reaction (PCR).</p><p><b>RESULTS</b>There were 1.41% samples caught by V.C., having a carrying rate highest in turtles of 8.9%. 14 strains were defined as three serogroups, and the numbers of Inaba, Ogawa, and Hikojima types were 2, 2, 10 respectively. Virulence genes had detected in 9 of 12 stains. All genes were detected in 5 strains, only ZOT genes in 3 strains, and both CTX and ACE genes in 1 strain.</p><p><b>CONCLUSIONS</b>Aquatic products from inshore in Zhejiang Province caught with V.C. strains might be divided into three serogroups. Most of them should be virulence genes. Cholera epidemic outbreak might be caused by those contaminated products.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Vibrio cholerae
/
China
/
Alimentos Marinhos
/
Fatores de Virulência
/
Microbiologia de Alimentos
/
Genes Bacterianos
/
Genética
/
Microbiologia
Tipo de estudo:
Estudo prognóstico
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2006
Tipo de documento:
Artigo
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