Construction of the recombinant adenovirus vector carrying antisense multidrug resistance (MDR1) gene / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 424-427, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-291097
ABSTRACT
The fragment of MDR1 gene obtained from the plasmid pHaMDR1-1 carrying the whole human MDR1 cDNA, was cloned reversely into the shuttle plasmid pAdTrack-CMV. With the resultant plasmid and the backbone plasmid pAdEasy-1, the homologous recombination took place in the Escherichia coli BJ5183 and the recombinant adenoviral plasmid was generated. The adenoviruses were packaged in the 293 cells. The recombinant adenovirus MDR1 vector would introduce the antisense MDR1 gene into the human multidrug resistance hepatocellular cell line effectively, which would provide an experimental basis for studies on the multidrug resistance in human hepatocellular carcinoma.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Plasmídeos
/
Recombinação Genética
/
Proteínas Recombinantes
/
Adenoviridae
/
Oligonucleotídeos Antissenso
/
Técnicas de Transferência de Genes
/
Carcinoma Hepatocelular
/
Membro 1 da Subfamília B de Cassetes de Ligação de ATP
/
Resistencia a Medicamentos Antineoplásicos
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2004
Tipo de documento:
Artigo
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