Construction of LACK gene recombinant plasmid and detection of its expression in eukaryotic cell / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 272-275, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-291132
ABSTRACT
The LACK gene from Leishmania, an analogue of the receptor of activated protein kinase C, was discovered recently. In this study, the LACK gene of Leishmania donovani was obtained from the recombinant plasmid T-LACK by PCR. The gene was cloned into eukaryotic expressed plasmid pcDNA3.1(+) to construct recombinant plasmid. This recombinant plasmid then was transfected into the eukaryotic cell COS-7, and the expression of LACK gene in eukaryotic cell was detected by RT-PCR and immunofluorescent staining. Both RT-PCR and immunofluorescent staining of recombinant plasmid transfected COS-7 showed positive reaction, thus indicating that the recombinant plasmid pcDNA3-LACK can express LACK protein in euka ryotic cell COS-7.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Leishmania donovani
/
Proteínas Recombinantes
/
DNA Recombinante
/
Transfecção
/
Proteínas de Protozoários
/
Clonagem Molecular
/
Células COS
/
Vacinas de DNA
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2004
Tipo de documento:
Artigo
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