Expression of quail vascular endothelial growth factor receptor quek-1 extracellular domain 2-4 loop cDNA in Pichia pastoris / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 157-167, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-291276
ABSTRACT
The extracellular domain 2-4 loop cDNA of quail vascular endothelial growth factor receptor quek1 (qVEGFR2) was obtained from plasmid carrying qVEGFR2 by PCR. Then it was cloned into expression vector pPICZalphaA of Pichia pastoris. To construct recombinant expression plasmid pPICZalphaA-qVEGFR2, linearized pPICZalphaA-qVEGFR2 with SacI was transformed to electroporated Pichia pastoris GS115. Subsequently, positive clone was selected by PCR and its phenotype was determined. SDSPAGE and Western blot assays of culture medium from a methanol-induced expression strain demonstrated that recombinant qVEGFR2 proteins were expressed and secreted into the culture medium. These results could provide a basis for further researches on tumor protein vaccine as well as for the preparation of tumor protein vaccine with Pichia pastoris.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pichia
/
Codorniz
/
Proteínas Recombinantes
/
Reação em Cadeia da Polimerase
/
Receptores de Neurotransmissores
/
DNA Complementar
/
Vacinas Anticâncer
/
Receptor 2 de Fatores de Crescimento do Endotélio Vascular
/
Vetores Genéticos
/
Genética
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS