Establishment of a real time quantitative-PCR assay for detection of TCR VgammaI-Jgamma gene rearrangement in acute lymphoblastic leukemia patients / 中华血液学杂志
Chinese Journal of Hematology
; (12): 425-428, 2004.
Article
em Zh
| WPRIM
| ID: wpr-291401
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WPRO
ABSTRACT
<p><b>OBJECTIVE</b>To improve the techniques for minimal residual disease (MRD) detection in acute lymphoblastic leukemia (ALL).</p><p><b>METHODS</b>A real time quantitative PCR method was established for quantifying the clonal TCRVgammaI-Jgamma gene rearrangement in 36 ALL patients.</p><p><b>RESULTS</b>The sensitivity of the established real time quantitative PCR was at 10(-4) level. The amount of TCRVgammaI-Jgamma gene rearrangement in newly diagnosed group, complete remission (CR) group and post hematopoietic stem cell transplantation (HSCT) group was (7.38 +/- 6.65) x 10(-2), (1.02 +/- 1.08) x 10(-2) and (3.89 +/- 5.65) x 10(-3) level, respectively. and the amount in newly diagnosed group was higher than that in CR group and HSCT group (P = 0.001). The MRD level of ALL patients in CR group was higher than that in HSCT group (P = 0.022). MRD can be detected in 6 ALL patients after HSCT, 2 of them with low MRD level (< 1 x 10(-3)) survived long disease-free survival, the other 4 with high MRD level relapsed within one year.</p><p><b>CONCLUSION</b>The established real time quantitative PCR assay is simple, rapid, sensitive and specific. Use of this assay to evaluate MRD in the remission ALL cases is helpful for prognosis prediction.</p>
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Índice:
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Assunto principal:
Patologia
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Prognóstico
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Terapêutica
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Região Variável de Imunoglobulina
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Reação em Cadeia da Polimerase
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Reprodutibilidade dos Testes
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Rearranjo Gênico da Cadeia gama dos Receptores de Antígenos dos Linfócitos T
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Leucemia-Linfoma Linfoblástico de Células Precursoras
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Genética
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Métodos
Tipo de estudo:
Diagnostic_studies
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Prognostic_studies
Limite:
Adolescent
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Adult
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Female
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Humans
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Male
Idioma:
Zh
Revista:
Chinese Journal of Hematology
Ano de publicação:
2004
Tipo de documento:
Article