Gene expression profile of yolk sac and fetal liver in mouse / 中华血液学杂志
Chinese Journal of Hematology
;
(12): 266-268, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-291452
ABSTRACT
<p><b>OBJECTIVE</b>To better understand the mechanisms of the fetal hematopoiesis turn over from primitive to definitive hematopoiesis through the expression level of c-kit(+) and sca-1(+), and major characters of gene expression profile of these cells.</p><p><b>METHODS</b>c-kit and sca-1 expression level were monitored with fluorescence activated cell sorting (FACS) of the mononuclear cells from mouse yolk sac and fetal liver, while gene expression profile was carried out with EST sequencing strategy.</p><p><b>RESULTS</b>The Sca-1(+) cells were increased while the c-kit(+) cells decreased with the embryonic development. Through profiling the functionally identified known genes, most of the highly expressed were globin genes, especially of embryonic types.</p><p><b>CONCLUSION</b>The erythropoiesis played a key role in early fetal hematopoiesis in mammalian.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fatores de Tempo
/
Saco Vitelino
/
Antígenos Ly
/
Diferenciação Celular
/
Embriologia
/
Regulação da Expressão Gênica no Desenvolvimento
/
Proteínas Proto-Oncogênicas c-kit
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Perfilação da Expressão Gênica
/
Biologia Celular
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Hematology
Ano de publicação:
2004
Tipo de documento:
Artigo
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