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Antibiotic resistance and molecular characterization of Vibrio cholera strains isolated from an outbreak of cholera epidemic in Jiangsu province / 中华预防医学杂志
Chinese Journal of Preventive Medicine ; (12): 128-131, 2015.
Artigo em Chinês | WPRIM | ID: wpr-291666
ABSTRACT
<p><b>OBJECTIVE</b>To assess the antibiotic resistance and molecular characterization of cholera strains and to provide basis for clinical treatment and prevention of cholera.</p><p><b>METHODS</b>4 stains isolated from an outbreak of cholera epidemic in Huai'an City in Jiangsu province in September 2010 were characterized using antibiotic susceptibility, biotype analysis, virluence genes detection, ctxB gene sequencing, and PFGE analysis.</p><p><b>RESULTS</b>The 4 strains were all resistant to sulphamethoxazole/trimethoprim, erythromycin, streptomycin. High drug susceptibility of the samples was found to 6 kinds of antibiotics such as amikacin, norfloxacin, ciprofloxacin, gentamicin, chloramphenicol, ampicillin. The isolates expressed phenotypic traits of both serogroup O1 ogawa and El Tor and carried 9 kinds of virulence genes, ctxA, ace, zot, toxR, tcpI, ompU, rtxC, tcpA, and hlyA gene. They were also identified as harboring the classical ctxB genotype based on amino acid residue substitutions. The PFGE profiles of NotI showed a single banding pattern, while SfiI's was 2 banding patterns.</p><p><b>CONCLUSION</b>The bacterium type of Vibrio cholerae causing the epidemic outbreak of cholera belonged to the atypical EL Tor variant which was also identified as toxicogenic strain. The mapping of the strains prompted that there should be the common contamination source. Drug sensitivity test can guide the clinical drug use, in order to reduce the emergence of resistant strains.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Vibrio cholerae / Virulência / Resistência Microbiana a Medicamentos / Cólera / Toxina da Cólera / Surtos de Doenças / Farmacorresistência Bacteriana / Vibrio cholerae O1 / Epidemias / Genótipo Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Preventive Medicine Ano de publicação: 2015 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Vibrio cholerae / Virulência / Resistência Microbiana a Medicamentos / Cólera / Toxina da Cólera / Surtos de Doenças / Farmacorresistência Bacteriana / Vibrio cholerae O1 / Epidemias / Genótipo Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Preventive Medicine Ano de publicação: 2015 Tipo de documento: Artigo