Characteristics of virulence gene in Vibrio parahaemolyticus strains isolated from clinical patients and environment in Hangzhou, China / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 200-203, 2004.
Artigo
em Chinês
| WPRIM
| ID: wpr-291788
ABSTRACT
<p><b>OBJECTIVES</b>To investigate the characteristics of virulence gene in Vibrio parahaemolyticus strains isolated from clinical patients and environment in Hangzhou, China.</p><p><b>METHODS</b>Thermostable direct hemolysin gene (tdh) and thermostable direct hemolysin-related hemolysin gene (trh) were determined in a total of 174 strains of V. parahaemolyticus isolated from patients and environment (seafood) in Hangzhou area by PCR.</p><p><b>RESULTS</b>The tdh was found in 92 out of 94 V. parahaemolyticus strains from food poisoning patients and in 33 out of 34 strains from sporadic diarrhea patients, and trh was not detected in all above clinical strains. Meanwhile the tdh was negative in all V. parahaemolyticus strains from environment, and the trh was also negative except one strain with urease activity. All strains with trh negative had no the activity of urease.</p><p><b>CONCLUSIONS</b>The V. parahaemolyticus strains from food poisoning patients and sporadic diarrhea patients are tdh positive and trh negative. The V. parahaemolyticus strains with tdh negative and almost trh positive in environment might be a potential pathogen in Hangzhou.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Frutos do Mar
/
Proteínas de Bactérias
/
Toxinas Bacterianas
/
Urease
/
Vibrioses
/
Vibrio parahaemolyticus
/
China
/
Classificação
/
Microbiologia Ambiental
/
Doenças Transmitidas por Alimentos
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2004
Tipo de documento:
Artigo
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