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Substance P up-regulates the TGF-beta 1 mRNA expression of human dermal fibroblasts in vitro / 中华整形外科杂志
Chinese Journal of Plastic Surgery ; (6): 234-236, 2002.
Artigo em Chinês | WPRIM | ID: wpr-292088
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of substance P in the formation of hypertrophic scar.</p><p><b>METHODS</b>Dermal fibroblasts derived from human normal skin were cultured with substance P alone or together with selective non-peptide NK-1 tachykinin antagonist, L-703, 606 oxalate salt. The effect of substance P on proliferation of fibroblasts was measured by MTT assay. Furthermore, the TGF-beta 1 mRNA expression in the fibroblasts was determined by in situ hybridization and image analysis.</p><p><b>RESULTS</b>Substance P enhanced fibroblast proliferation dose-dependently, which showed the maximum rate when the concentration of substance P was 25 ng/ml or higher in the culture media. By 48 hours cultured with 25 ng/ml of substance P, the fibroblasts expressed TGF-beta 1 mRNA more significantly than the fibroblasts without substance P. The effects of substance P on both fibroblast proliferation and TGF-beta 1 mRNA expression could be antagonized by L-703, 606 oxalate salt.</p><p><b>CONCLUSION</b>The results suggest that substance P may play an important role in phenotype changes of fibroblasts in skin scarring.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Quinuclidinas / RNA Mensageiro / Substância P / Expressão Gênica / Divisão Celular / Regulação para Cima / Células Cultivadas / Fator de Crescimento Transformador beta / Derme Idioma: Chinês Revista: Chinese Journal of Plastic Surgery Ano de publicação: 2002 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Farmacologia / Quinuclidinas / RNA Mensageiro / Substância P / Expressão Gênica / Divisão Celular / Regulação para Cima / Células Cultivadas / Fator de Crescimento Transformador beta / Derme Idioma: Chinês Revista: Chinese Journal of Plastic Surgery Ano de publicação: 2002 Tipo de documento: Artigo