Substance P up-regulates the TGF-beta 1 mRNA expression of human dermal fibroblasts in vitro / 中华整形外科杂志
Chinese Journal of Plastic Surgery
;
(6): 234-236, 2002.
Artigo
em Chinês
| WPRIM
| ID: wpr-292088
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the role of substance P in the formation of hypertrophic scar.</p><p><b>METHODS</b>Dermal fibroblasts derived from human normal skin were cultured with substance P alone or together with selective non-peptide NK-1 tachykinin antagonist, L-703, 606 oxalate salt. The effect of substance P on proliferation of fibroblasts was measured by MTT assay. Furthermore, the TGF-beta 1 mRNA expression in the fibroblasts was determined by in situ hybridization and image analysis.</p><p><b>RESULTS</b>Substance P enhanced fibroblast proliferation dose-dependently, which showed the maximum rate when the concentration of substance P was 25 ng/ml or higher in the culture media. By 48 hours cultured with 25 ng/ml of substance P, the fibroblasts expressed TGF-beta 1 mRNA more significantly than the fibroblasts without substance P. The effects of substance P on both fibroblast proliferation and TGF-beta 1 mRNA expression could be antagonized by L-703, 606 oxalate salt.</p><p><b>CONCLUSION</b>The results suggest that substance P may play an important role in phenotype changes of fibroblasts in skin scarring.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Quinuclidinas
/
RNA Mensageiro
/
Substância P
/
Expressão Gênica
/
Divisão Celular
/
Regulação para Cima
/
Células Cultivadas
/
Fator de Crescimento Transformador beta
/
Derme
Idioma:
Chinês
Revista:
Chinese Journal of Plastic Surgery
Ano de publicação:
2002
Tipo de documento:
Artigo
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