Expression of the hemagglutinin and neuramidinase gene of influenza A virus H1N1 in Pichia methanolica / 生物工程学报
Chinese Journal of Biotechnology
;
(12): 1068-1073, 2010.
Artigo
em Chinês
| WPRIM
| ID: wpr-292170
ABSTRACT
On the basis of successful cloning the full length hemagglulinin (HA) and neuramidinase (NA) gene and sequence analysis of influenza virus H1N1, part of the gene was ligated into pMETA. Expression vectors pMETA/HA (52-1 557 bp) and pMETA/NA (121-1 263 bp) were constructed and expressed in pMAD16 induced by methanol. Recombinant protein was purified through Ni2+ affinity chromatography. Western blotting and ELISA were used to determine the antigenic activity of the recombinant protein. SDS-PAGE showed that the recombinant capsid gene could be overexpressed in Pichia methanolica. ELISA and Western blotting showed that the recombinant protein had antigenicity.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Pichia
/
Proteínas Recombinantes
/
Clonagem Molecular
/
Alergia e Imunologia
/
Vírus da Influenza A Subtipo H1N1
/
Vetores Genéticos
/
Genética
/
Hemaglutininas
/
Metabolismo
/
Neuraminidase
Idioma:
Chinês
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2010
Tipo de documento:
Artigo
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