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Cloning of keratinocyte growth factor 2 gene (KGF2) and its transformation to Brassica napus L / 生物工程学报
Chinese Journal of Biotechnology ; (12): 767-771, 2010.
Artigo em Chinês | WPRIM | ID: wpr-292210
ABSTRACT
Recently, more research about the plant bioreactor expressing genes encoding human proteins was reported. In the present study, the cDNA of the human gene keratinocyte growth factor 2 (KGF2) was replaced with plant preferred codons by PCR, and the modified full-length cDNA was cloned into the plant expression vector pCAMBIA-YO containing the oil-body promoter. The fusion construct pCAMBIA-YO-KGF2 was transformed into Brassica napus by Agrobacterium tumefacien-mediated cotyledon transformation method. The transgenic seedlings were identified by PCR, Southern and western blot analysis all showed that KGF2 gene was successfully expressed in in transgenic Brassica napus.
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Rhizobium / Transformação Genética / Plantas Geneticamente Modificadas / Clonagem Molecular / DNA Complementar / Brassica napus / Fator 7 de Crescimento de Fibroblastos / Vetores Genéticos / Genética / Metabolismo Tipo de estudo: Estudo prognóstico Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Rhizobium / Transformação Genética / Plantas Geneticamente Modificadas / Clonagem Molecular / DNA Complementar / Brassica napus / Fator 7 de Crescimento de Fibroblastos / Vetores Genéticos / Genética / Metabolismo Tipo de estudo: Estudo prognóstico Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Biotechnology Ano de publicação: 2010 Tipo de documento: Artigo