Construction of plasmid expression vector for specific peptide of the rubella virus E1 gene / 中华男科学杂志
National Journal of Andrology
;
(12): 318-321, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-292379
ABSTRACT
<p><b>OBJECTIVE</b>To construct a recombinant plasmid vector of the RV specific fragment for expressing the specific fragment of RV E1 protein.</p><p><b>METHODS</b>RNA of the RV attenuated live vaccine Wistar RA27/3 strain was extracted and reversely transcribed. The specific fragment of the E1 gene was amplified and the PCR products cloned in the vector pGEX-2T after purification. Positive clones were selected and identified by two-enzyme digestion and sequence analysis.</p><p><b>RESULTS</b>A 330 bp target fragment was successfully cloned, and the sequence of the recombinant plasmid was consistent with the original sequence.</p><p><b>CONCLUSION</b>Successful cloning of the RV El specific fragment and the construction of the recombinant plasmid have laid a foundation for further expressing the recombinant protein.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Plasmídeos
/
Vírus da Rubéola
/
RNA Viral
/
Dados de Sequência Molecular
/
Sequência de Bases
/
Expressão Gênica
/
Proteínas do Envelope Viral
/
Clonagem Molecular
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Alergia e Imunologia
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2009
Tipo de documento:
Artigo
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