Molecular pathways of germ cell apoptosis following testicular torsion in rats / 中华男科学杂志
National Journal of Andrology
;
(12): 144-148, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-292408
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the molecular mechanism of germ cell apoptosis following testicular torsion in rats.</p><p><b>METHODS</b>Healthy male Sprague-Dawley rats (n = 16) were equally randomized into a control and a torsion group and the models of testicular torsion (720 degrees 2 h) were established. Twenty-four hours later, the apoptosis and count of germ cells were determined by flow cytometry, the expressions of Bax, Fas and Fas ligand (FasL) mRNA semiquantitatively analyzed by RT-PCR and the cytochrome C release detected by Western blot.</p><p><b>RESULTS</b>Compared with the control group, there was an obvious increase in the number of apoptotic germ cells, a marked decrease in that of haploid and tetraploid cells and significantly up-regulated expressions of Bax and Fas/FasL mRNA in the torsion group (P <0.01). The Western blot analysis showed that the cytochrome C release was remarkably increased 24 hours after the detorsion. There were significant differences between the two groups (P <0.01).</p><p><b>CONCLUSION</b>There are two major signaling pathways of cell apoptosis following testicular torsion, intercellular and intracellular. Up-regulated expressions of the apoptosis-related molecules Bax and Fas/FasL and increased cytochrome C release may play an important role in germ cell apoptosis following testicular torsion in rats.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Torção do Cordão Espermático
/
RNA Mensageiro
/
Ratos Sprague-Dawley
/
Apoptose
/
Receptor fas
/
Biologia Celular
/
Modelos Animais de Doenças
/
Proteína X Associada a bcl-2
/
Proteína Ligante Fas
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2009
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS