Grape seed extract induces morphological changes of prostate cancer PC-3 cells / 中华男科学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To observe the morphological changes of prostate cancer PC-3 cells induced by grape seed extract (GSE).</p><p><b>METHODS</b>PC-3 cells were incubated with different concentrations of GSE (100, 200 and 300 microg/ml) for 24, 48 and 72 hours, and then observed for morphological changes by invert microscopy, HE staining and transmission electron microscopy.</p><p><b>RESULTS</b>The incubated PC-3 cells appeared round, small, wrinkled and broken under the invert microscope and exhibited the classical morphological characteristics of cell death under the electron microscope, including cell atrophy, increased vacuoles, crumpled nuclear membrane, and chromosome aggregation.</p><p><b>CONCLUSION</b>GSE can cause morphological changes and induce necrosis and apoptosis of PC-3 cells.</p>