Establishment of human embryonic stem cell line NJGLLhES1 / 中华男科学杂志
National Journal of Andrology
;
(12): 1083-1089, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-292449
ABSTRACT
<p><b>OBJECTIVE</b>To report the derivation and characterization of a new human embryonic stem cell (hESC) line NJGLLhES1.</p><p><b>METHODS</b>From the inner cell mass of frozen-thawed human embryos and with ICR mouse embryonic fibroblasts as the feeder layer, we established a new human embryonic stem cell line, which was named NJGLLhES1. We detected the karyotype of the cell line, determined the expressions of alkaline phosphatase, the specific cell surface antigens SSEA-3, SSEA-4, TRA-1-60, TRA-1-81 and the marker gene Oct-4, and examined the formation of embryoids and teratomas.</p><p><b>RESULTS</b>NJGLLhES1 was maintained for over 1 year in vitro, with the morphological characteristics of hESC, a normal karyotype, positive expressions of alkaline phosphatase and specific cell marker genes, and the potential of forming embryoids and teratomas.</p><p><b>CONCLUSION</b>A new human embryonic stem cell line NJGLLhES1 was successfully established, which remains karyotypically and phenotypically stable, undifferentiated and capable of self-renewal and pluripotential differentiation.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Diferenciação Celular
/
Linhagem Celular
/
Técnicas de Cultura de Células
/
Biologia Celular
/
Embrião de Mamíferos
/
Células-Tronco Embrionárias
/
Camundongos Endogâmicos ICR
Limite:
Animais
/
Humanos
Idioma:
Chinês
Revista:
National Journal of Andrology
Ano de publicação:
2008
Tipo de documento:
Artigo
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