Expression, purification and application of EsxB protein in Staphylococcus aureus / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 364-366, 2012.
Artigo
em Chinês
| WPRIM
| ID: wpr-292465
ABSTRACT
<p><b>OBJECTIVE</b>This study aimed to establish the method of expression and purification of EsxB protein, explore the EsxB antibody-positive Staphylococcus aureus (S. aureus) clinical infection status and relevance of drug resistance.</p><p><b>METHODS</b>Constructed EsxB prokaryotic expression system by homologous recombination, Ni(2+) column was used to purify EsxB protein; and then ELISA was used to detect the anti-EsxB antibodies in serum of 78 patients with S. aureus infection; antimicrobial susceptibility of related S. aureus strains by automatic bacterial identification analyzer.</p><p><b>RESULTS</b>EsxB prokaryotic protein expression system was constructed and EsxB protein was purified successfully; anti-EsxB antibodies were present in the serum of patients with S. aureus infection up to 28.21% (22/78). The proportion of multi-drug resistant and Methicillin-resistant S. aureus strains isolated from anti-EsxB antibodies positive patients were 100.0% (22/22), 77.3% (17/22), respectively, which were statistically higher than those strains isolated from anti-EsxB antibody-negative patients (35.7% (20/56) and 21.4% (12/56), respectively) (all P values < 0.01).</p><p><b>CONCLUSION</b>Method for expression and purification of EsxB protein was established. All the S. aureus strains isolated from EsxB antibody-positive patients were multidrug resistant strains and most of them were resistant to methicillin.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Staphylococcus aureus
/
Proteínas de Bactérias
/
Testes de Sensibilidade Microbiana
/
Staphylococcus aureus Resistente à Meticilina
/
Genética
/
Meticilina
/
Antibacterianos
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2012
Tipo de documento:
Artigo
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