Establishment and verification of dose effect relation curves between T cell receptor gene mutation frequency and ionizing radiation dose / 中华预防医学杂志
Chinese Journal of Preventive Medicine
;
(12): 1090-1092, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-292534
ABSTRACT
<p><b>OBJECTIVE</b>To establish the dose-effect curve between TCR MF and ionizing radiation.</p><p><b>METHODS</b>Peripheral lymphocytes were collected from 8 healthy adults (4 males and 4 females) and cultured in vitro with 12 well culture plates. They were stimulated by PHA-P and IL-2 after exposed to different doses of irradiation (0.00 - 8.00 Gy) and cultured for 7 d. The dose-effect curve was established after measuring TCR MF using flow cytometry. Also, using the same method, we separated and cultured the peripheral lymphocytes collected from 16 radiotherapy cancer patients, whose radiation styles and doses were different, and then measured TCR MF to estimate the whole equivalent dose of radiotherapy patients through the dose-effect curve. Peripheral blood was collected and cultured, chromosome aberration (dicentric and ring) was determined under microscope to estimate irradiation dose.</p><p><b>RESULTS</b>The relationship of dose-effect between the TCR MF and ionizing radiation (0.00 - 8.00 Gy) was well, the curve of large dose group (2.00 - 8.00 Gy), low dose group (0.00 - 1.00 Gy) and 0.00 - 8.00 Gy dose group were met with the quadratic polynomial model, the equation was TCR MF = -32.8579 + 20.5436D + 0.6341D(2), TCR MF = 1.796 + 0.017D + 5.155D(2) and TCR MF = -0.6229 + 6.305D + 0.6919D(2), respectively. D was the radiation dose (Gy). Using the established curve and the chromosome aberration method to estimate the systemic exposure dosage, the average relative deviation was 16.8%.</p><p><b>CONCLUSION</b>The curve established by the TCR gene mutation analysis technology can be applied to exposure dose estimation of victims in ionization radiation accidents.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Efeitos da Radiação
/
Radiação Ionizante
/
Receptores de Antígenos de Linfócitos T
/
Estudos de Casos e Controles
/
Genes Codificadores dos Receptores de Linfócitos T
/
Relação Dose-Resposta à Radiação
/
Taxa de Mutação
/
Genética
Tipo de estudo:
Estudo observacional
Limite:
Feminino
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
Chinese Journal of Preventive Medicine
Ano de publicação:
2011
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS