Experimental study on dog's bone marrow stem cells transfected by pIRES2-EGFP-IGF-1 gene / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 739-742, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-292975
ABSTRACT
<p><b>OBJECTIVE</b>To establish the bone marrow stem cells (MSC) model which could highly express the insulin-like growth factor 1 (IGF-1) transfected by dog's IGF-1 gene.</p><p><b>METHODS</b>pIRES2-EGFP-IGF-1 was transfected into MSC by lipofectamine. Positive clones were selected with G418. The expression of IGF-1 protein in the MSC was determined by immunohistochemistry and Western blot analysis. The IGF-1 in the supernatant of the transfected MSC was detected by sandwich-in ELISA. The periodontal ligament cells (PDLC) were cultured in the supernatant of the transfected MSC. The changes of PDLC' proliferation were observed by MTT.</p><p><b>RESULTS</b>IGF-1-transfected MSC could apparently express IGF-1. The IGF-1 protein in the supernatant of the transfected MSC was confirmed by sandwich-in ELISA. IGF-1 could promote the PDLC' proliferation.</p><p><b>CONCLUSIONS</b>The MSC transfected by dog's IGF-1 gene can highly express IGF-1, which may lay the foundation for further study on periodontal regeneration.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fator de Crescimento Insulin-Like I
/
Células da Medula Óssea
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Transfecção
/
Células Cultivadas
/
Biologia Celular
/
Células-Tronco Mesenquimais
/
Vetores Genéticos
/
Genética
/
Metabolismo
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2006
Tipo de documento:
Artigo
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