Mutation and polymorphism of the ameloblastin gene in ameloblastomas / 中华口腔医学杂志
Chinese Journal of Stomatology
;
(12): 678-681, 2006.
Artigo
em Chinês
| WPRIM
| ID: wpr-293011
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the presence of mutation or polymorphism of ameloblastin (AMBN) gene in ameloblastomas.</p><p><b>METHODS</b>Genomic DNA was extracted from frozen tissues of 10 ameloblastomas and one malignant ameloblastoma. AMBN gene alterations were detected by PCR-direct sequencing. Restriction fragment length polymorphism (RFLP) analysis was used to further determine the nature of the changes in AMBN detected in tumor samples in comparison to 100 control samples.</p><p><b>RESULTS</b>AMBN mutation was not identified in all 11 tumor samples. The 7 types of AMBN gene alteration identified in 9 cases were proven to be polymorphisms, three of which were not previously reported. The frequency of genotype and allele of the three SNPs complied with the Hardy-Weinberg equilibrium. Neither genotype nor allele frequency showed a significant association with ameloblastoms.</p><p><b>CONCLUSIONS</b>AMBN gene mutation is not identified in the present group of ameloblastomas. The frequently detected AMBN alterations in ameloblastomas are polymorphisms, which appear to be unrelated to the occurrence of ameloblastomas.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Polimorfismo Genético
/
Ameloblastoma
/
Neoplasias Maxilomandibulares
/
Estudos de Casos e Controles
/
Análise de Sequência de DNA
/
Proteínas do Esmalte Dentário
/
Genética
/
Mutação
Tipo de estudo:
Estudo observacional
/
Fatores de risco
Limite:
Adolescente
/
Adulto
/
Criança
/
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Stomatology
Ano de publicação:
2006
Tipo de documento:
Artigo
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