Exosomes derived form bladder transitional cell carcinoma cells induce CTL cytotoxicity in vitro / 中华肿瘤杂志
Chinese Journal of Oncology
;
(12): 738-741, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-293063
ABSTRACT
<p><b>OBJECTIVE</b>To isolate and purify exosomes derived from human bladder transitional cell carcinoma T24 cells, analyze the morphology and protein composition, and investigate the antitumor effect of specific cytotoxic T lymphocytes induced by exosomes.</p><p><b>METHODS</b>Exosomes were isolated and purified by ultrafiltration and sucrose gradient centrifugation, and characterized by electron microscopy and Western blot. Dendritic cells were amplified and purified from peripheral blood and pulsed with exosomes. Then they were co-cultured with T cells, and divided into 3 groups exosome-pulsed DC group, unplused DC group and control group. Alamar-Blue assay was used to evaluate the specific cytolytic activity.</p><p><b>RESULTS</b>The exosomes were in size about 30 approximately 90 nm saucer-shaped membranous vesicles. HSP70, ICAM-1 and CK20 were detected by Western blot. The CTL induced by DC pulsed with exosomes had significant cytolytic activity (P < 0.01).</p><p><b>CONCLUSION</b>The exosomes derived from T24 cells are loaded with immunoprotein HSP70 and ICAM-1, and DC pulsed with exosomes can promote the anti-tumor effect of CTLs in vitro.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Neoplasias da Bexiga Urinária
/
Células Dendríticas
/
Ativação Linfocitária
/
Carcinoma de Células de Transição
/
Linfócitos T
/
Linfócitos T Citotóxicos
/
Molécula 1 de Adesão Intercelular
/
Proteínas de Choque Térmico HSP70
/
Técnicas de Cocultura
Limite:
Humanos
Idioma:
Chinês
Revista:
Chinese Journal of Oncology
Ano de publicação:
2009
Tipo de documento:
Artigo
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