Study on effect of berberine on modulating lipid and CPT I A gene expression / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 2715-2718, 2011.
Artigo
em Chinês
| WPRIM
| ID: wpr-293233
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the modulating effect on lipid and gene expressions of CPT I A caused by berberine (Ber) in experimental hyperlipidemia rats.</p><p><b>METHOD</b>Male SD rats were randomly divided into 5 groups according to the blood lipid values normal group, hyperlipidemia group, 300 mg x kg(-1) x d(-1) Ber-treated group, 60 mg x kg(-1) x d(-1) Ber-treated group, and 7.2 mg x kg(-1) x d(-1) lovastatin-treated group. Normal group were fed with base diet and other groups were fed with high fat and cholesterol diet. 12 weeks after drugs were given the TC, TG, LDL-C, and HDL-C from rat blood samples were tested by automatic biochemistry analyzer. Gene expressions of CPT I A and PPARalpha were evaluated by RT-PCR and Western blot, respectively.</p><p><b>RESULT</b>It was shown that Ber significantly decreased TC and LDL-C, but increased HDL-C in dose-dependent manner, elevated expressions of CPT I A mRNA and protein without influence on PPARalpha expression. Similar effects from lovastatin on lipidemia were observed except the Ber effect on CPT I A gene expression.</p><p><b>CONCLUSION</b>Ber has modulating effect on the lipid metabolism, the mechanism of which may be by promoting the CPT I A gene expression.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Berberina
/
Medicamentos de Ervas Chinesas
/
Carnitina O-Palmitoiltransferase
/
Distribuição Aleatória
/
Expressão Gênica
/
Ratos Wistar
/
PPAR gama
/
Modelos Animais de Doenças
/
Tratamento Farmacológico
/
Metabolismo dos Lipídeos
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Animais
/
Humanos
/
Masculino
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2011
Tipo de documento:
Artigo
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