Matrine-induced erythroid differentiation of K562 cells is associated with activation of the apoptotic pathway / 南方医科大学学报
Journal of Southern Medical University
;
(12): 478-480, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-293347
ABSTRACT
<p><b>OBJECTIVE</b>To observe matrine-induced erythroid differentiation of K562 cells in relation to activation of the apoptotic pathway in vitro.</p><p><b>METHODS</b>K562 cells were cultured in the presence or absence of matrine at different concentrations for 4 days, and the morphological and ultramicrostructural changes of the cells were observed using inverted microscopy and transmission electron microscopy, respectively. The expression of apoptosis-related protein p27kip1 was detected by immunocytochemistry.</p><p><b>RESULTS</b>Compared to untreated K562 cells, the cells treated with matrine at 0.10 g/L exhibited apoptostic characteristics in the cellular morphology and ultramicrostructure, with the expression of p27kip1 protein upregulated in a concentration- and time-dependent manner.</p><p><b>CONCLUSION</b>Matrine-induced erythroid differentiation of K562 cells is associated with cell apoptosis, and upregulation of p27kip1 protein expression may play a crucial role in the process of apoptosis.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Farmacologia
/
Fisiologia
/
Quinolizinas
/
Fatores de Tempo
/
Imuno-Histoquímica
/
Transdução de Sinais
/
Leucemia Eritroblástica Aguda
/
Apoptose
/
Células K562
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2008
Tipo de documento:
Artigo
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