Tumor-specific T cell recptor gene transfection promotes memory T cell differentiation in vitro / 南方医科大学学报
Journal of Southern Medical University
;
(12): 313-316, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-293391
ABSTRACT
<p><b>OBJECTIVE</b>To investigate effect of tumor-specific T cell receptor gene transfection on memory T cell differentiation in vitro.</p><p><b>METHODS</b>TCRVbeta7.1 gene was transferred into peripheral blood mononuclear cells (PBMCs) obtained from healthy adults, and the expression of Vbeta7.1 was detected by flow cytometry before and after the transfection. Memory T cell differentiation was induced by stimulation with the hepatocarcinoma cell line BEL-7402 in vitro. The expression of surface molecules CD45RO, CD45RA and CCR7 was analyzed by flow cytometry to identify the phenotype and subsets of the memory T cells. Fluorescence-activated cell sorting was performed to detect the apoptosis of the tumor cells, and enzyme-linked immunoabsorbent assay was used to determine the production of interferon-gamma (IFN-gamma) for assessing the immune function of the memory T cells.</p><p><b>RESULTS</b>Flow cytometry showed that TCRVbeta7.1 gene was efficiently expressed after transfection. After stimulation by the tumor cells in vitro, the expression of CD45RO in TCRVbeta7.1 gene-modified T cells increased gradually, and analysis of the coexpression of CD45RA and CCR7 revealed that the effector memory T cells constituted the majority of the differentiated memory T cells. The apoptotic rate of the tumor cells induced by the T cells increased significantly with also obviously increased INF-gamma secretion in the memory T cells.</p><p><b>CONCLUSION</b>Tumor-specific TCRVbeta7.1 gene transfection can promote the differentiation of the memory T cells, the majority of which belongs to effector memory T cells that perform immune functions by inducing apoptosis and cytokine secretion.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Ensaio de Imunoadsorção Enzimática
/
Linfócitos T
/
Transfecção
/
Diferenciação Celular
/
Células Cultivadas
/
Interferon gama
/
Apoptose
/
Antígenos Comuns de Leucócito
/
Carcinoma Hepatocelular
Limite:
Adulto
/
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2008
Tipo de documento:
Artigo
Similares
MEDLINE
...
LILACS
LIS