Detection of multidrug resistance-associated proteins MRP2, MRP3, and MRP5 mRNA expressions in hepatocarcinoma cells using SYBR real-time PCR / 南方医科大学学报
Journal of Southern Medical University
;
(12): 219-221, 2008.
Artigo
em Chinês
| WPRIM
| ID: wpr-293412
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the differential expression of genes encoding multidrug resistance-associated protein 2 (MRP2), MRP3 and MRP5 in human normal liver cell line L-02, hepatocarcinoma cell line BEL and its adriamycin-resistant counterpart BEL/ADM cell line.</p><p><b>METHODS</b>The total RNA was extracted from L-02, BEL and BEL/ADM cells, respectively, and the mRNAs were reversely transcribed into cDNA. The expression levels of MRP2, MRP3, MRP5 mRNAs were detected according to the standard curve using real-time fluorescence quantitative PCR.</p><p><b>RESULTS</b>The expression of MRP2 mRNA in BEL/ADM cell line was significantly higher than that in L-02 and BEL cells (P=0.000), and the latter two cells showed similar expression levels of MRP2 mRNA (P=0.468). The expressions of MRP3 and MRP5 mRNA showed statistically significant difference between the 3 cell lines (P=0.000).</p><p><b>CONCLUSION</b>MRP2 might play a role in the intrinsic drug resistance, and MRP3 and MRP5 are related to the acquired drug resistance of hepatocellular carcinoma.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
RNA Mensageiro
/
Regulação Neoplásica da Expressão Gênica
/
Carcinoma Hepatocelular
/
Reação em Cadeia da Polimerase Via Transcriptase Reversa
/
Proteínas Associadas à Resistência a Múltiplos Medicamentos
/
Linhagem Celular Tumoral
/
Metabolismo
/
Proteínas de Neoplasias
Tipo de estudo:
Estudo diagnóstico
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Southern Medical University
Ano de publicação:
2008
Tipo de documento:
Artigo
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