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Expression, genetic and epigenetic alterations of LTF gene in nasopharyngeal carcinoma cell lines / 中华肿瘤杂志
Chinese Journal of Oncology ; (12): 729-733, 2010.
Artigo em Chinês | WPRIM | ID: wpr-293494
ABSTRACT
<p><b>OBJECTIVE</b>To investigate the expression of LTF mRNA in several nasopharyngeal cancer (NPC) cell lines, and analyze the relationship between the genetic and epigenetic changes and expression of LTF gene.</p><p><b>METHODS</b>The expression level of LTF was detected in NPC cell lines HNE1, HNE2, HNE3, CNE1, CNE2, 5-8F, 6-10B cells and tissues of 15 cases of chronic nasopharyngitis by RT-PCR. The LTF protein level was analyzed by Western blotting in 6-10B cells. Then LOH, mutation and methylation status of LTF was examined by microsatellites analysis, PCR-SSCP, MSP and bisulfite genomic sequencing, respectively.</p><p><b>RESULTS</b>15 chronic nasopharyngitis tissues showed stable LTF expression, while there were weak expression in 6-10B cells and absent expression in remaining detected NPC cell lines. There was a significantly lower LTF expression in chronic nasopharyngitis tissues (Z = -3.738, P = 0.000). No LTF protein expression was observed in 6-10B cells. LOH analysis demonstrated that allele loss of LTF wasn't found in NPC cell lines. LTF mutation was noted in 14.3% (1/7) of NPC cell lines. DNA sequencing confirmed the mutation point in the promoter region (-305 bp to -50 bp) was at -218 bp (del T) of LTF gene in the HNE1 cell line. Methylation of LTF gene was not found in chronic nasopharyngitis. However, methylation of LTF promoter was detected in all NPC cell lines. LTF mRNA expression was increased in 5-8F and 6-10B cell lines after treatment with 5-aza-2-deoxycytidine.</p><p><b>CONCLUSION</b>There is an inactivation of expression of LTF gene in the NPC cell lines. Its molecular mechanism may be related with methylation of promoter region and deletion mutation.</p>
Assuntos
Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Azacitidina / RNA Mensageiro / Neoplasias Nasofaríngeas / Nasofaringite / Regiões Promotoras Genéticas / Deleção de Genes / Metilação de DNA / Perda de Heterozigosidade Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2010 Tipo de documento: Artigo

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Texto completo: DisponíveL Índice: WPRIM (Pacífico Ocidental) Assunto principal: Patologia / Farmacologia / Azacitidina / RNA Mensageiro / Neoplasias Nasofaríngeas / Nasofaringite / Regiões Promotoras Genéticas / Deleção de Genes / Metilação de DNA / Perda de Heterozigosidade Limite: Humanos Idioma: Chinês Revista: Chinese Journal of Oncology Ano de publicação: 2010 Tipo de documento: Artigo