Studies on fingerprinting of Flos Buddleja by RP-HPLC / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish fingerprinting of Flos Buddleja by using RP-HPLC for the quality control.</p><p><b>METHOD</b>The HPLC condition was as follows Inertsil ODS-3 C18 analytical column (4.6 mm x 250 mm, 5 microm), gredient eluation with MeCN (0.1% TFA)-H2O (0.1%TFA), flow rate 1.0 mL x min(-1), detection wavelength 254 nm. 10 commercial samples were analyzed to establish a fingerprinting.</p><p><b>RESULT</b>Among the obtained fingerprinting, most of the detected peaks were separated effectively. The accuracy, repeatability and stability of this method were satisfied. The RSDs of relative retention time and area of aimed peaks which existed in all samples wereless than 5%. Theresults were in accordance with the request of fingerprinting.</p><p><b>CONCLUSION</b>The established fingerprinting can be used for the quality control of Flos Buddleja.</p>