Effect of factors on callus biomass and synthetic mass of hypericin in Hypericum perforatum / 中国中药杂志

ABSTRACT

<p><b>OBJECTIVE</b>To study the effect of several factors on the quantity of hypericin in H. perforatum callus.</p><p><b>METHOD</b>High efficiency liquid phase chromatography and plant tissue culture were applied.</p><p><b>RESULT AND CONCLUSION</b>When the ratio of nitro-nitrogen to amina-nitrogen is 31, the callus biomass is 1.6-fold and the synthetic mass of hypericin rises. 0.1-0.20 mg x L(-1) mannose improves the content of total hypericin. The addition of PVP or PVPP can promote improvement of the growth and biosynthesis of callus.</p>