An approach to screen nephrotoxic components in traditional Chinese medicine based on fluorescent probe and HK-2 cells and its application / 中国中药杂志
China Journal of Chinese Materia Medica
;
(24): 1577-1580, 2013.
Artigo
em Chinês
| WPRIM
| ID: wpr-294065
ABSTRACT
Using fluorescein diacetate labeled HK-2 cells, a fast method for screening nephrotoxic components in traditional Chinese medicines (TCMs) was proposed in this study. The methodology validation showed that the linearity, stability and accuracy of the proposed method were suitable for screening nephrotoxic components in vitro . This method was further applied on screen 352 components from 32 Chinese Pharmacopoeia-indexed toxic TCMs. The results indicated that 31 components from 14 toxic TCMs, including Badou, had significant toxicity on HK-2 cells, which suggested these components may cause nephrotoxicity. The components from the other 18 toxic TCMs had no significant toxicity on HK-2 cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Bioensaio
/
Medicamentos de Ervas Chinesas
/
Linhagem Celular
/
Química
/
Toxicidade
/
Corantes Fluorescentes
/
Rim
/
Métodos
Limite:
Humanos
Idioma:
Chinês
Revista:
China Journal of Chinese Materia Medica
Ano de publicação:
2013
Tipo de documento:
Artigo
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