Enhancing alkylating agent resistance through ERCC2 gene transfection in human glioma cell line / 中华医学杂志(英文版)
Chinese Medical Journal
;
(24): 1171-1174, 2003.
Artigo
em Inglês
| WPRIM
| ID: wpr-294140
ABSTRACT
<p><b>OBJECTIVE</b>To confirm the enhancing effect of excision repair cross complementing rodent repair deficiency gene 2 (ERCC2) on alkylating agents resistance.</p><p><b>METHODS</b>The authors constructed a pcDNA3-ERCC2 plasmid. The pcDNA3-ERCC2 was transfected into a selected ERCC2 negative human glioma cell line, SKMG-4, using liposome-mediated transfection. After G418 selection, a stable transfected cell line was obtained and tested for cytotoxicity of several alkylating agents.</p><p><b>RESULTS</b>The stable transfectant was obtained and confirmed by RT-PCR as well as Western blot analysis to be strongly expressing ERCC2 at both mRNA and protein levels. The IC(90) ( micro mol/L) of two alkylating agents, cisplatin and melphalan, increased from 1.0 to 1.75 (75%) and 5.6 to 9.0 (61%), respectively, compared with control cell line.</p><p><b>CONCLUSION</b>The present data provided evidences and confirmed the authors' previous results that ERCC2 contributes, at least partially, to alkylating agent resistance in human glioma cell line.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fatores de Transcrição
/
Células Tumorais Cultivadas
/
Transfecção
/
Proteínas
/
Cisplatino
/
DNA Helicases
/
Resistencia a Medicamentos Antineoplásicos
/
Antineoplásicos Alquilantes
/
Proteínas de Ligação a DNA
Limite:
Humanos
Idioma:
Inglês
Revista:
Chinese Medical Journal
Ano de publicação:
2003
Tipo de documento:
Artigo
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