Rapid testing on drug susceptibility of Mycobacterium tuberculosis with nitrate reducrase assay / 中华流行病学杂志

ABSTRACT

<p><b>OBJECTIVE</b>To establish a rapid method for testing drug sussceptibility on Mycobacterium tuberculosis.</p><p><b>METHODS</b>Taking absolute Concentration method for drug susceptibility testing of M. tuberculosis as the "gold standard", we examined the drug-resistant of M. tuberculosis strain with nitrate reducrase assay (NRA) and the drug-resistant of M. tuberculosis germ in sputum with NRA.</p><p><b>RESULTS</b>NRA and absolute concentration method was basically comparable with NRA susceptibility as 96.5% and the specificity was 100%, When comparing with traditional absolute concentration method, NRA could shorten the time about 3 weeks. Using NRA to test the drug-resistant of M. tuberculosis germ in sputum, its susceptibility was more than 66.7% and specificity was 100%, within 10-20 days.</p><p><b>CONCLUSION</b>NRA could be used as a rapid drug susceptibility testing on M. tuberculosis.</p>