Effect and mechanism of jianpi qinghua recipe on renal functions of adriamycin-induced nephropathic rats from the angle of inhibiting renal fibrosis / 中国中西医结合杂志
Chinese Journal of Integrated Traditional and Western Medicine
;
(12): 733-738, 2014.
Artigo
em Chinês
| WPRIM
| ID: wpr-294405
ABSTRACT
<p><b>OBJECTIVE</b>To explore the effect of Jianpi Qinghua Recipe (JQR) on renal functions of adriamycin-induced focal segmental glomerular sclerosis (FSGS) rats from the angle of activating fibroblasts to myofibroblast (MyoF).</p><p><b>METHODS</b>Totally 56 rats were randomly divided into the normal control group (n=8), the sham-operation group (n =8), and the model group (n=40). The FSGS rat model was induced by nephrectomy of left kidney plus intravenous injection of adriamycin. Successfully modeled rats were further divided into 5 groups, i.e., the model group, the JQR group, the JPR (Jianpi Recipe) group, the QHR (Qinghua Recipe) group, and the NDQ (Niaoduqing) group, 8 in each group. Corresponding drugs were administered to rats in all groups, 2 mL each time, for 56 days. The effect of JQR on serum creatinine (SCr), urea nitrogen, 24-h urinary protein excretion, a-smooth muscle actin (alpha-SMA) mRNA, collagen type III (Col III) mRNA, fibronectin (FN) mRNA, and collagen type IV (Col IV) mRNA were observed.</p><p><b>RESULTS</b>JQR could significantly lower SCr, urea nitrogen, and 24-h urinary protein excretion levels (P < 0.01), and significantly decrease mRNA levels of alpha-SMA, Col III, FN, and Col IV (P < 0.01). It was advantageous over the NDQ group. Compared with JPR, the relative expression levels of Col III mRNA and FN mRNA of JQR and QHR were significantly lower (P < 0.01).</p><p><b>CONCLUSIONS</b>JQR could improve the renal function and renal fibrosis in the adriamycin-induced nephropathic model rats. Its efficacy was superior to that of NDQ. Its mechanisms might be linked with inhibiting activation of fibroblasts.</p>
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fibrose
/
Medicamentos de Ervas Chinesas
/
Doxorrubicina
/
Ratos Sprague-Dawley
/
Usos Terapêuticos
/
Modelos Animais de Doenças
/
Tratamento Farmacológico
/
Toxicidade
/
Rim
/
Nefropatias
Limite:
Animais
Idioma:
Chinês
Revista:
Chinese Journal of Integrated Traditional and Western Medicine
Ano de publicação:
2014
Tipo de documento:
Artigo
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