Spaceflight alters the gene expression profile of cervical cancer cells / 癌症
Chinese Journal of Cancer
;
(12): 842-852, 2011.
Artigo
em Inglês
| WPRIM
| ID: wpr-294451
ABSTRACT
Our previous study revealed that spaceflight induced biological changes in human cervical carcinoma Caski cells. Here, we report that 48A9 cells, which were subcloned from Caski cells, experienced significant growth suppression and exhibited low tumorigenic ability after spaceflight. To further understand the potential mechanism at the transcriptional level, we compared gene expression between 48A9 cells and ground control Caski cells with suppression subtractive hybridization (SSH) and reverse Northern blotting methods, and analyzed the relative gene network and molecular functions with the Ingenuity Pathways Analysis (IPA) program. We found 5 genes, SUB1, SGEF, MALAT-1, MYL6, and MT-CO2, to be up-regulated and identified 3 new cDNAs, termed B4, B5, and C4, in 48A9 cells. In addition, we also identified the two most significant gene networks to indicate the function of these genes using the IPA program. To our knowledge, our results show for the first time that spaceflight can reduce the growth of tumor cells, and we also provide a new model for oncogenesis study.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Patologia
/
Voo Espacial
/
Regulação Neoplásica da Expressão Gênica
/
Biblioteca Gênica
/
Regulação para Cima
/
Neoplasias do Colo do Útero
/
Northern Blotting
/
Perfilação da Expressão Gênica
/
Linhagem Celular Tumoral
/
Proliferação de Células
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
Chinese Journal of Cancer
Ano de publicação:
2011
Tipo de documento:
Artigo
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