The effect of co-immobilized TNF-alpha/IFN-gamma on mitochondrial membrane potential of HeLa cells / 生物医学工程学杂志
Journal of Biomedical Engineering
;
(6): 972-977, 2009.
Artigo
em Chinês
| WPRIM
| ID: wpr-294529
ABSTRACT
This study inquired into the mechanisms of co-immobilized cytokines and free cytokines-induced apoptosis on HeLa cells. With the use of photochemical fixed method, TNF-alpha/IFN-gamma were co-immobilized on a 24-well polystyrene culture plate. HeLa cells were stained with fluorescent probe JC-1 to detect the changes of mitochondrial membrane potential (deltapsim), and then were examined by flow cytometry. The results showed that co-immobilized cytokines could induce the apoptosis of HeLa cells in a dose-independent manner. When treated with low-dose of co-immobilized cytokines (20ng/ml), the mitochondrial membrane potential (deltapsim) of HeLa cells continually decreased in 6 days. These indicate that low dose co-immobilized cytokines have a long-term of apoptosis-inducing effect on HeLa cells. We assume that there is close relationship between the mitochondrial membrane potential decrease and the apoptosis of HeLa cells.
Texto completo:
DisponíveL
Índice:
WPRIM (Pacífico Ocidental)
Assunto principal:
Farmacologia
/
Fisiologia
/
Células HeLa
/
Interferon gama
/
Fator de Necrose Tumoral alfa
/
Apoptose
/
Relação Dose-Resposta a Droga
/
Membranas Mitocondriais
/
Potencial da Membrana Mitocondrial
/
Proteínas Imobilizadas
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2009
Tipo de documento:
Artigo
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